- Ambassador (Bug Ambassador, Fingerprint Ambassador)
- Double Agent/Suspected Double Agent (Contact Double Agent)
- Seduction Target (Seduce Target)
Any playable character can fill any of the cast roles. Characters are chosen at random by the game to be suspected double agents, although some maps don't have them even when the Contact Double Agent mission is selected. All other roles are cast by the spy. Roles whose missions are not selected will not be available to be cast and will not appear in the game. No character can play more than one role.
Casting choices may reflect the spy's personality and personal preferences, which introduces a bias that a savvy sniper can exploit. The sniper has an advantage if they know, for example, that the spy likes to play as one of the Danger Brothers, or that they prefer their seduction target to be a young female. To eliminate this bias, many spies simply select the first (randomly chosen) character offered by the game for each role. While waiting for the spy to select the game options, the sniper can watch to see how long the spy spends casting roles; this can be an indication of whether or not the spy is consciously selecting their cast.